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Tris-HCL緩沖液(1mol,pH7.5)的詳細(xì)介紹
更新時間：2023-10-19   點擊次數(shù)：1100次

 Tris-HCL緩沖液(1mol,pH7.5)的詳細(xì)介紹


【產(chǎn)品名稱】 ：Tris-HCL緩沖液(1mol,pH7.5)

【產(chǎn)品規(guī)格】 ：500ml                     

【儲存條件】 ：RT                

【有效期】 ：12個月                 

【產(chǎn)品簡介】 ：

Tris(三羥甲基氨基甲烷)為弱堿，分子式為C4H11NO3，

相對分子量為121.14, 在25℃下pKa為8.1，Tris緩

沖液的有效緩沖范圍在pH7.0～9.2之間，Tris堿的水

溶液pH在10.5左右，一般加入鹽酸調(diào)節(jié)pH值至所需值，

即可獲得該pH值的緩沖液，常用pH值為6.8、7.4、8.0、

8.8，其pH值隨溫度變化很大，一般來說溫度每升高1℃，

pH值下降0.03。1M Tris-HCl pH8.0是分子生物學(xué)中最常

用的試劑之一，用來作為各種緩沖液的基礎(chǔ)成分，調(diào)節(jié)酸堿

度，例如配制Tris-EDTA溶液，TBE電泳緩沖液等。

 

Tris-HCl緩沖液(1mol/L,pH7.5)采用進(jìn)口Tris、去離子水

配制而成，pH值約為7.5，未經(jīng)15psi高壓滅菌處理，被廣

泛用作核酸和蛋白質(zhì)的溶劑。

 


本產(chǎn)品僅供科研實驗用，不做其它用途！

 

【操作步驟】僅供參考 ：

1、根據(jù)實驗具體要求操作。

 

【注意事項】 ：

1、對人體有刺激性，請注意適當(dāng)防護。

2、為了您的安全和健康，請穿實驗服并戴一次性手套操作。

 Tris-HCL緩沖液(1mol,pH7.5)的詳細(xì)介紹


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