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苯丙氨酸解氨酶(PAL)檢測試劑盒(苯丙氨酸微板法)的注意事項
更新時間：2023-12-15   點擊次數(shù)：338次

苯丙氨酸解氨酶(PAL)檢測試劑盒(苯丙氨酸微板法)的注意事項

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苯丙氨酸解氨酶( L- phenylalanine ammonia-lyase，PAL)是催化直接脫掉 L-苯丙氨酸上的氨而生成反式桂皮酸的酶，該酶多存在于高等植物、酵母、菌類可溶性部分物質，是1961 年J.Koukol在大麥中發(fā)現(xiàn)的，推測其分子量約為 30萬，這是一個可把苯丙氨酸用于酚類化合物合成的酶。在組織中的活性可隨外界因素而發(fā)生顯著變化，用光照、病傷害、植物激素處理等會使活性顯著增加，在多數(shù)情況下在組織中活性增加時，酶發(fā)生失活作用，這時組織中具有活性酶的量很快就會減少,據(jù)認為這種失活是與類蛋白質物質作用有關，測定細胞木質素合成途徑中間代謝物及關鍵酶活性 可以探討多種生物細胞發(fā)育過程中木質素沉積的代謝機理，為減少水果石細胞含量提高其品質提供依據(jù)。苯丙氨酸解氨酶(PAL)檢測試劑盒(苯內氨酸微板法)檢測原理是以苯丙氨酸作為底物，在酶促反應的最適條件下采用每隔一定時間測定產(chǎn)物生成量的方法，于酶標儀290nm處檢測吸光度，以吸光度變化所需酶量進行計算，主要用于植物組織的裂解液或勻漿液、血清等樣品中內源性的苯丙氨酸解氨酶活性，尤其適用于檢測水果中苯丙氨酸解氨酶活性。該試劑盒僅用于科研領域，不適用于臨床診斷或其他用途。

 

 苯丙氨酸解氨酶(PAL)檢測試劑盒(苯丙氨酸微板法)的注意事項


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