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鏈球菌蛋白G SPG使用說(shuō)明
更新時(shí)間：2024-01-30   點(diǎn)擊次數(shù)：341次

鏈球菌蛋白G SPG使用說(shuō)明

簡(jiǎn)介：蛋白G是G、C型鏈球菌細(xì)胞壁中的一種蛋白，能特異性的同免疫球蛋白IgG分子的Fc段結(jié)合而不影響其Fab段與抗原分子結(jié)合的能力。它不僅能與人、小鼠、大鼠IgG 的全部亞型相結(jié)合，還可與豚鼠、兔、山羊、牛、綿羊、馬等的IgG相結(jié)合，明顯優(yōu)于只能結(jié)合人、小鼠IgG的蛋白A。蛋白G的用途極為廣泛，可用于從血清中分離并純化或去除IgG、單克隆抗體的純化、抗原抗體復(fù)合物的分離等。

蛋白G與瓊脂糖凝膠結(jié)合后還可制備用于抗體純化的親和填料，從腹水、血清、細(xì)胞培養(yǎng)液上清或細(xì)胞抽提物中分離和純化不同亞型的IgG。

天然蛋白G除含有IgG結(jié)合域之外，同時(shí)還具有白蛋白和細(xì)胞表面結(jié)合域。重組蛋白G去除了白蛋白和細(xì)胞表面結(jié)合域，以減少非特異性結(jié)合，比天然蛋白G和蛋白A具有更大的親和力。

別名：蛋白G（Protein G）

來(lái)源：大腸桿菌

性狀：凍干粉

凍干前體系：5mM PB、PH7.4

分子量：28kDa

等電點(diǎn)（PI）：4.79

純度：≥95%（SDS-PAGE檢測(cè)）

使用方法：無(wú)菌水配制，現(xiàn)用現(xiàn)配

規(guī)格：1mg、10mg、100mg、500mg

保存：-20℃


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