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重組耐堿性蛋白A (r ATPA)價格

重組耐堿性蛋白A (r ATPA)價格

產品時間:2024-07-31

簡要描述:

重組耐堿性蛋白A (r ATPA)價格:重組耐堿性蛋白A(r-ATPA)是一種通過基因工程技術對天然金黃色葡萄球菌蛋白A進行改造后獲得的重組蛋白。作為重要的科研試劑,r-ATPA廣泛應用于生物化學、免疫學、分子生物學等領域的科研實驗中。

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重組耐堿性蛋白A (r ATPA)價格


簡介:重組耐堿蛋白A是在天然金黃色葡萄球菌蛋白A的基礎上對蛋白質結構進行了改造。同天然Protein A相比,r-ATPA去除了與免疫球蛋白Fab區(qū)的結合位點,增強了其與Fc區(qū)結合的占一行,同時提高了蛋白質的穩(wěn)定性和耐堿性。

將r-ATPA與瓊脂糖凝膠結合后可制備用于抗體純化的親和填料,從腹水、血清、細胞培養(yǎng)液上清或細胞抽提物中分離和純化多種哺乳動物不同亞型的抗體或包含抗體Fc片段的基因工程重組蛋白。

特性與優(yōu)勢

耐堿性:與天然蛋白A相比,r-ATPA顯著提高了對堿性環(huán)境的耐受性,能夠在> 0.1 M NaOH的條件下保持穩(wěn)定。

專一性增強:通過去除與Fab的結合位點,r-ATPA增強了與Fc片段結合的專一性,使其更適用于抗體純化和相關研究。

穩(wěn)定性提高:改造后的r-ATPA在結構和功能上都更加穩(wěn)定,有利于長期保存和使用。


應用領域

抗體純化:r-ATPA與瓊脂糖凝膠鍵合后可制備用于抗體純化的親和填料,能夠從腹水、血清、細胞培養(yǎng)上清或細胞抽提物中分離和純化多種哺乳動物不同亞型的抗體或包含抗體Fc片段的基因工程重組蛋白。

科研實驗:作為重要的科研試劑,r-ATPA廣泛應用于生物化學、免疫學、分子生物學等領域的科研實驗中。


別名:重組耐堿蛋白A(Recombinant-Alkali tolerant Protein A)

來源:大腸桿菌

性狀:凍干粉

凍干前體系:5mM PB、PH7.4

分子量:28kDa

等電點(PI):5.24

純度:≥95%(SDS-PAGE檢測)

使用方法:無菌水配制,現(xiàn)用現(xiàn)配

規(guī)格:1mg、10mg、100mg、500mg

保存: -20℃

有效期:1年


注意事項

避免變性:在使用過程中應避免使用強酸、強堿、強氧化劑、高濃度的有機溶劑等易引起蛋白質變性的試劑。

專用性:r-ATPA為科研實驗專用試劑,不可用于臨床治療或其他非科研用途。


重組耐堿性蛋白A (r ATPA)價格

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